Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

dos Santos, Guilherme Brzoskowski; da Silva, Edileuza Danieli; Kitano, Eduardo Shigueo; Battistella, Maria Eduarda; Monteiro, Karina Mariante; de Lima, Jeferson Camargo; Ferreira, Henrique Bunselmeyer; Serrano, Solange Maria de Toledo; Zaha, Arnaldo

doi:10.1186/s13071-022-05232-8

Parasites & Vectors

Table 3 Identification and relative abundance of proteins present in HF samples from E. granulosus and E. ortleppi bovine pulmonary hydatid cysts

From: Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

Accession number^a	Protein name	Molecular Mass^b	EG		EO
Accession number^a	Protein name	Molecular Mass^b	NSAF	SD	NSAF	SD
EgrG_000144400	Abnormal EMBroygenesis family member emb 9	168 kDa	0.00382	0.00227	0.00254	0.00064
EgrG_000061200	Actin	42 kDa	0.01910	0.01909	0.01722	0.00630
EgrG_000156400	Aldo keto reductase family 1 member B4	42 kDa	0.00199	0.00101	0.00277	0.00102
EgrG_000704400	Alpha-mannosidase	118 kDa	0.01298	0.00567	0.01019	0.00120
EgrG_000530400	Amine oxidase	84 kDa	0.00932	0.00324	0.00531	0.00204
EgrG_001032200	Aminotransferase class III; Ornithine aminotransferase	46 kDa	0.01093	0.00898	0.00551	0.00226
EgrG_000184900	Antigen 5	55 kDa	0.04773	0.00409	0.05786	0.02534
EgrG_000575900	Basement membrane specific heparan sulfate	860 kDa	0.01233	0.00292	0.00869	0.00066
EgrG_000701800	Basement membrane specific heparan sulfate	96 kDa	0.00857	0.00187	0.00467	0.00036
EgrG_000879900	Beta D xylosidase 2	92 kDa	0.00252	0.00108	0.00271	0.00146
EgrG_000789900	Beta mannosidase	108 kDa	0.00220	0.00084	0.00115	0.00032
EgrG_000903100	Calsyntenin 1	130 kDa	0.00405	0.00445	0.00285	0.00157
EgrG_000970500	Cathepsin D lysosomal aspartyl protease	47 kDa	0.01626	0.00742	0.01783	0.00509
EgrG_000144350	Collagen alpha 1(IV) chain	172 kDa	0.00553	0.00266	0.00351	0.00066
EgrG_000417600	Collagen alpha 1(IV) chain	182 kDa	0.00280	0.00195	0.00154	0.00066
EgrG_000203400	Collagen alpha 1(V) chain	172 kDa	0.00630	0.00263	0.00512	0.00185
EgrG_000144300	Collagen alpha 1(V) chain	177 kDa	0.00453	0.00246	0.00309	0.00012
EgrG_000729300	Collagen alpha 1(XV) chain	191 kDa	0.00398	0.00232	0.00250	0.00054
EgrG_000823800	Collagen alpha 2(I) chain	131 kDa	0.00914	0.00326	0.00605	0.00239
EgrG_001190600	Collagen type I II III V XI alpha	123 kDa	0.00786	0.00374	0.00445	0.00175
EgrG_000524200	Collagen type XI alpha 2	163 kDa	0.00445	0.00130	0.00191	0.00115
EgrG_000766600	Cysteine-rich secretory protein LCCL domain-containing; Peptidase inhibitor 16	29 kDa	0.01633	0.01258	0.00751	0.00292
EgrG_000255800	EGF domain protein	267 kDa	0.00385	0.00187	0.00193	0.00014
EgrG_000682900	Epididymal secretory protein E1; Niemann Pick C2 protein	20 kDa	0.00778	0.00123	0.00369	0.00151
EgrG_000824000	Estrogen regulated protein EP45	45 kDa	0.01048	0.00136	0.00722	0.00093
EgrG_001061900	Expressed conserved protein	74 kDa	0.00762	0.00602	0.01483	0.00569
EgrG_000412500	Expressed conserved protein	14 kDa	0.01324	0.00948	0.01267	0.00320
EgrG_000523100	Expressed conserved protein	53 kDa	0.00638	0.00255	0.00600	0.00175
EgrG_000596300	Expressed conserved protein	25 kDa	0.01830	0.01295	0.01610	0.00609
EgrG_000316400	Expressed protein	35 kDa	0.00261	0.00088	0.00217	0.00114
EgrG_000842900	Fgfr protein	80 kDa	0.00302	0.00128	0.00273	0.00042
EgrG_001060700	Fibrillar collagen chain FAp1 alpha	116 kDa	0.00989	0.00164	0.00725	0.00357
EgrG_000176400	Fras1 related extracellular matrix protein	263 kDa	0.00187	0.00107	0.00147	0.00037
EgrG_000905600	Fructose-bisphosphate aldolase	40 kDa	0.01739	0.00955	0.01265	0.00119
EgrG_000712600	Gynecophoral canal protein	97 kDa	0.00883	0.00348	0.00577	0.00146
EgrG_000824400	Gynecophoral canal protein; Transforming growth factor-beta-induced protein ig-h3	73 kDa	0.01180	0.00181	0.00923	0.00139
EgrG_000422350	Hemicentin 1	477 kDa	0.00279	0.00100	0.00138	0.00065
EgrG_001132400	Laminin	395 kDa	0.00113	0.00081	0.00054	0.00029
EgrG_000458400	Laminin subunit gamma	163 kDa	0.00254	0.00085	0.00105	0.00044
EgrG_000684200	Lipid transport protein N terminal	344 kDa	0.00439	0.00189	0.00236	0.00186
EgrG_000343000	Neurogenic locus notch protein	339 kDa	0.00555	0.00140	0.00384	0.00030
EgrG_001181950	Papilin	67 kDa	0.00315	0.00094	0.00159	0.00057
EgrG_000920600	Peptidyl-prolyl cis–trans isomerase	17 kDa	0.01078	0.00123	0.01451	0.00122
EgrG_000292700	Phosphoenolpyruvate carboxykinase	71 kDa	0.01145	0.00131	0.01344	0.00424
EgrG_001132700	Poly(U) specific endoribonuclease	29 kDa	0.00748	0.00254	0.00659	0.00084
EgrG_000849600	Proteinase inhibitor I25 cystatin	31 kDa	0.03216	0.01174	0.02514	0.01025
EgrG_001133400	Protein-L-isoaspartate O-methyltransferase	27 kDa	0.00630	0.00374	0.00363	0.00209
EgrG_000929500	SPONdin extracellular matrix glycoprotein	111 kDa	0.00293	0.00178	0.00126	0.00016
EgrG_000381200	Tapeworm specific antigen B (AgB8/1)	10 kDa	0.09772	0.00805	0.19553	0.08363
EgrG_000791700	Thioredoxin peroxidase	21 kDa	0.01305	0.00927	0.01254	0.00268
EgrG_001060600	Type II collagen B	154 kDa	0.00352	0.00080	0.00206	0.00116
EgrG_000317300	Vesicular amine transporter	49 kDa	0.01186	0.00156	0.00688	0.00342

The listed proteins were identified in the three biological replicates from each species. Quantitative data are presented based on averaged NSAF values calculated for E. granulosus (EG) and E. ortleppi (EO)
NSAF Normalized spectral abundance factor, SD standard deviation
^aAccording to E. granulosus genome annotation (PRJEB121, version WBPS11) available at WormBase ParaSite
^bMolecular mass calculated from primary sequence
Top ten NSAF values in HF samples of each species are highlighted in bold

Back to article page

ISSN: 1756-3305

Contact us

Submission enquiries: journalsubmissions@springernature.com