Table 1 Primer sequences of cytokines and fibrosis related factors
Cytokines | Primers | Sequence |
|---|---|---|
18S | Forward | 5ʹ-TGCACCACCAACTGCTTAGC-3ʹ |
Reverse | 5ʹ-GTGGTCATGAGCCCTTCCA-3ʹ | |
IFN-γ | Forward | 5ʹ-GATGCATTCATGAGTATTGCCAAGT-3ʹ |
Reverse | 5ʹ-GTGGACCACTCGGATGAGCTC-3ʹ | |
IL-4 | Forward | 5ʹ-AGATCATCGGCATTTTGAACG-3ʹ |
Reverse | 5ʹ-TTTGGCACATCCATCTCCG-3ʹ | |
TGF-β1 | Forward | 5ʹ-CTTTAGGAAGGACCTGGGTT-3ʹ |
Reverse | 5ʹ-CAGGAGCGCACAATCATGTT-3ʹ | |
Col-1 | Forward | 5ʹ-GCGAGTGCTGTGCTTTCTG-3ʹ |
Reverse | 5ʹ-TACCTCGACTCCTACATCTTC-3ʹ | |
Col-3 | Forward | 5ʹ-CCCAACCCAGAGATCCCATT-3ʹ |
Reverse | 5ʹ-GAAGCACAGGAGCAGGTGTAGA-3ʹ | |
HMGB1 | Forward | 5ʹ-CGGATGCTTCTGTCAACTTCTC-3ʹ |
Reverse | 5ʹ-GTTTCTTCGCAACATCACCAAT-3ʹ | |
IL-13 | Forward | 5ʹ-GCCAGCCCACAGTTCTACAGC-3ʹ |
Reverse | 5ʹ-GTGATGTTGCTCAGCTCCTCA-3ʹ |