Table 1 Methodological comparison of RPA-PfAgo and nested PCR with Sanger sequencing
Haplotype | Method | Sensitivity (%) | Specificity (%) | False negative (%) | False positive (%) | |||
|---|---|---|---|---|---|---|---|---|
Nested PCR with Sanger sequencing | RPA-PfAgo | N (%) | 95% CI | N (%) | 95% CI | |||
CVMNK | 55 | 55 | 100 | 0.9187–1 | 100 | 0.8587–1 | 0.00 | 0.00 |
CVIET | 19 | 19 | 100 | 0.7908–1 | 100 | 0.9315–1 | 0.00 | 0.00 |
CV M/I N/E K/T | 11 | 11 | 100 | 0.6786–1 | 100 | 0.9385–1 | 0.00 | 0.00 |
Total | 85 | 85 | 100 | 0.9461–1 | 100 | NaN | 0.00 | 0.00 |