Fig. 3

TAU reduced the expression of inflammatory factors in DCs and RAW264.7 cells. A Relative transcription levels of Nlrp3 in DCs treated with 25 μM ART, TAU or the solvent control. B Relative transcription levels of AIM2 in DCs treated with 25 μM ART, TAU or the solvent control. C Relative transcription levels of NOD2 in DCs treated with 25 μM ART, TAU or the solvent control. D Relative transcription levels of caspase-1 in DCs treated with 25 μM ART, TAU or the solvent control. E Relative transcription levels of Nlrp3 in RAW264.7 cells treated with 25 μM ART, TAU, or the solvent control. F Relative transcription levels of AIM2 in RAW264.7 cells treated with 25 μM ART, TAU or the solvent control. G Relative transcription levels of NOD2 in RAW264.7 cells treated with 25 μM ART, TAU or the solvent control. H Relative transcription levels of caspase-1 in RAW264.7 cells treated with 25 μM ART, TAU or the solvent control. I Expression levels of the proteins NLRP3, IL-1β and pro-IL-1β in DCs treated with 25 μM ART, TAU or 1% ethanol. J Relative expression levels of NLRP3 in DCs after 24 h of treatment with 25 μM ART, TAU or 1% ethanol. K Relative expression levels of IL-1β in DCs after 24 h of treatment with 25 μM ART, TAU or 1% ethanol. L Relative expression levels of pro-IL-1β in DCs after 24 h of treatment with 25 μM ART, TAU or 1% ethanol. M Expression levels of the NLRP3, IL-1β and pro-IL-1β proteins in RAW264.7 cells treated with 25 μM ART, TAU or 1% ethanol for 24 h. N Expression levels of NLRP3 in RAW264.7 cells treated with 25 μM ART, TAU, or 1% ethanol for 24 h. O Relative expression levels of IL-1β in RAW264.7 cells treated with 25 μM ART, TAU or 1% ethanol for 24 h. P Relative expression levels of pro-IL-1β in RAW264.7 cells treated with 25 μM ART, TAU or 1% ethanol for 24 h. n = 3 biological replicates. P-values, shown above bars, were calculated using the Kruskal‒Wallis test. The data represent the mean ± standard deviation of the mean. AIM2, Absent in melanoma 2 gene; ART, artemisinin; IL, interleukin; DCs, dendritic cells; iRBCs, P. berghei ANKA-infected red blood cells; mRNA, messenger RNA; NOD2, nucleotide binding oligomerization domain containing 2 gene, Nlrp3, NLRP3, NOD-, LRR- and pyrin domain-containing protein 3 gene, protein; TAU, taurine