Mosquito mutations F290V and F331W expressed in acetylcholinesterase of the sand fly Phlebotomus papatasi (Scopoli): biochemical properties and inhibitor sensitivity

Table 1 Kinetics of AcSCh hydrolysis of wild type and mutant rPpAChEs

	WT	G119S	F290V	F331W
K_m ± SEM (n = 3), µM	123 ± 25a	156 ± 16a	367 ± 25b	474 ± 68b
Vmax ± SEM (n = 3), µmol/min/mg	74 ± 6.7a	7.7 ± 2.7c	35 ± 1.2b	16 ± 2.3c
Relative V_max/K_m	1.0	0.082	0.158	0.056

Letters next to each kinetic value indicate results of ANOVA with Tukey’s means separation test, where values labeled by different letters are significantly different (P < 0.05 or less). SEM standard error of the mean

ISSN: 1756-3305