Haemoproteus tartakovskyi and Plasmodium relictum (Haemosporida, Apicomplexa) differentially express distinct 18S rRNA gene variants in bird hosts and dipteran vectors

Table 1 Primers and probes used for analysis of P. relictum SGS1 differential 18S rRNA gene expression

Primer/probe	Description	Targeted 18S variant^a	Primer/probe sequences (5′–3′)	Annealing temp (°C)
SGS1_Q160_F	Forward primer	v1, v2	GCT CGT AGT TGA ATT TCA AAG	53.6
SGS1_Q160_R	Reverse primer	v1, v2	TCA TCA CAT AAA GCA ACG AAG	54.6
SGS1-Pr1_Q	TaqMan qPCR probe	v1	FAM-GTG TTA AAT ATA GTG CTT CGG CTT ATA TTT TCC ACA TTT C-TAMRA	63.0
SGS1-Pr2_Q	TaqMan qPCR probe	v2	FAM-GAT ACG TGT TAA ATG GTG CTT CGG C-TAMRA	62.9
SGS1-Pr1_ISH	CISH probe	v1	GAA ATG TGG AAA ATA TAA GCC GAA GCA CTA TAT TTA ACA C-digoxigenin	63.0
SGS1-Pr2_ISH	CISH probe	v2	GCC GAA GCA CCA TTT AAC ACG TAT C-digoxigenin	62.9
SGS1_Q1_F	Forward dye-based qPCR primer	v1	AGC GAA TAC AAT ATC AGA TAT GTG	54.8
SGS1_Q1_R	Reverse dye-based qPCR primer	v1	CCT GTT TTA TAA ACC GTC TGC	55.2
SGS1_Q2_F	Forward dye-based qPCR primer	v2	CTT TAT CGG ATA CGT GTT AAA TGG	56.0
SGS1_Q2_R	Reverse dye-based qPCR primer	v2	TTA GCT GTT TTA AAC TGT TTG CT	55.5

ISSN: 1756-3305