Haemoproteus tartakovskyi and Plasmodium relictum (Haemosporida, Apicomplexa) differentially express distinct 18S rRNA gene variants in bird hosts and dipteran vectors

Table 2 Primers and probes used for analysis of H. tartakovskyi SISKIN1 differential 18S rRNA expression

Primer/probe	Description	Targeted 18S variant^a	Primer/probe sequences (5′–3′)	Annealing temp (°C)
SISKIN1_Q190_F	Primer	v1, v2, v3	AGA TCA GAG ACC TTT GTA ATC C	55.0
SISKIN1_Q190_R	Primer	v1, v2, v3	CCC AAA ATC TCG GAT TTC TC	54.1
SISKIN1-Pr1_Q	TaqMan qPCR probe	v1	FAM-CTT GGA GAA CAT CGC CCG AGG CTA G-TAMRA	64.9
SISKIN1-Pr2_Q	TaqMan qPCR probe	v2	FAM-AGA CGG AGT GAG CTT CCT ATG ACG TT-TAMRA	65.4
SISKIN1-Pr3_Q	TaqMan qPCR probe	v3	FAM-GAG TTG CTG TCT TAT AAC GAT CGC ATG C-TAMRA	64.7
SISKIN1-Pr1_ISH	CISH probe	v1	CTA GCC TCG GGC GAT GTT CTC CAA G-Digoxigenin	64.9
SISKIN1-Pr2_ISH	CISH probe	v2	AAC GTC ATA GGA AGC TCA CTC CGT CT-Digoxigenin	65.4
SISKIN1-Pr3_ISH	CISH probe	v3	GCA TGC GAT CGT TAT AAG ACA GCA ACT C-Digoxigenin	64.7

ISSN: 1756-3305