Table 1 Primer pairs used for the amplification of F. hepatica ITS-1, cox1, nad1 and microsatellite loci
Gene/locus | Primer sequence (5′-3′) | Fluorescent dye | References |
|---|---|---|---|
ITS-1 | ITS1-F: 5′-TTGCGCTGATTACGTCCCTG-3′ | – | Itagaki et al. [43] |
ITS1-R: 5′-TTGGCTGCGCTCTTCATCGAC-3′ | |||
cox1 | Ita8: 5′-ACGTTGGATCATAAGCGTGT-3′ | – | Itagaki et al. [43] |
Ita9: 5′-CCTCATCCAACATAACCTCT-3′ | |||
nad1 | Ita10: 5′-AAGGATGTTGCTTTGTCGTGG-3′ | – | Itagaki et al. [43] |
Ita2: 5′-GGAGTACGGTTACATTCACA-3′ | |||
nad1a | Nad1_for: 5′-TGTTGCAGAGGTTTGCGGA-3′ | – | This study |
Nad1_rev: 5′-CCCCCAGAAATACCGACGAA-3′ | |||
Fh_2 | F: 5′-TGAGAAACTGATTCACCGACTG-3′ | ATTO565 | Cwiklinski et al. [78] |
R: 5′-GAGCTTGTGCTCTCGGAACTA-3′ | |||
Fh_5 | F: 5′-CATCACCACTGTCTTCGATCA-3′ | FAM | Cwiklinski et al. [78] |
R: 5′-CGAAGCATTGATAAGATTTCCA-3′ | |||
Fh_6 | F: 5′-ACGTCCGTCCGTTAAGTGAG-3′ | ATTO550 | Cwiklinski et al. [78] |
R: 5′-TTTGAGGTCGACATCCTTCA-3′ | |||
Fh_10 | F: 5′-TTTAGTCGCGGAGCTACCAT-3′ | ATTO532 | Cwiklinski et al. [78] |
R: 5′-CCACTTTCGTCATGCACATT-3′ | |||
Fh_11 | F: 5′-TAAACCGTTGCTTCACGTTG-3′ | ATTO550 | Cwiklinski et al. [78] |
R: 5′-CAAAGTGTTTGGCGAGCTG-3′ | |||
Fh_12 | F: 5′-CCACGAGAAGTGGAATTCGT-3′ | ATTO532 | Cwiklinski et al. [78] |
R: 5′-GTAGGTCCACTCCCTGTCCA-3′ | |||
Fh_13 | F: 5′-GAAACTGTCCCGAAAACGAG-3′ | FAM | Cwiklinski et al. [78] |
R: 5′-GCGTGCAACATAGGTGAAAA-3′ | |||
Fh_15 | F: 5′-AATGCGGAAAAGAGCGATTA-3′ | ATTO565 | Cwiklinski et al. [78] |
R: 5′-GAAATTGGGAGCAACTGCAT-3′ |