Fig. 4

Detection of Wolbachia in adult male D. immitis by immunohistochemistry with anti-WSP antibody. The rectangular box indicates 10× magnification. Scale bar 100 µM. A–C Cross-section from groups 1, 2, and 3 (control groups necropsied on 0, 30, and 60 days post-treatment), respectively. Dense Wolbachia clusters (arrows) in the lateral cord. D–F Cross-sections from groups 4, 5, and 6 (DOXY 5 mg/kg, 7.5 mg/kg, and 10 mg/kg, necropsied 30 days post-treatment), respectively. Scattered Wolbachia (arrows) in lateral cord. G–I Cross-sections from groups 7, 8, and 9 (DOXY 5 mg/kg, 7.5 mg/kg, and 10 mg/kg, necropsied 60 days post-treatment), respectively. Single Wolbachia (arrows) in the lateral cord