Table 2 Real-time PCR data summary of relative Wolbachia levels in adult female D. immitis. Percentages were calculated using the ΔΔCt method
Percentage of Wolbachia in adult female D. immitis | ||||
|---|---|---|---|---|
Days post-treatment | Group | DOXY | % median | % interquartile range (IQR) |
0 | 1 | Control | 81.8 | (72.1, 96.3) |
30 | 2 | Control | 109.9 | (89.9, 136.8) |
4 | 5 mg/kg | 11.1 | (7.4, 17.3) | |
5 | 7.5 mg/kg | 9.6 | (6.8, 14.4) | |
6 | 10 mg/kg | 9.2 | (7.1, 11.3) | |
60 | 3 | Control | 88 | (75.8, 99.1) |
7 | 5 mg/kg | 1.3 | (1.0, 2.1) | |
8 | 7.5 mg/kg | 0.9 | (0.9, 1.0) | |
9 | 10 mg/kg | 0.8 | (0.5, 1.6) | |