Table 3 Real-time PCR data summary of relative Wolbachia levels in adult male D. immitis. Percentages were calculated using the ΔΔCt method
Percentage of Wolbachia in adult male D. immitis | ||||
|---|---|---|---|---|
Days post-treatment | Group | DOXY | % Median | % Interquartile range (IQR) |
0 | 1 | Control | 101.4 | (67.9, 130.4) |
30 | 2 | Control | 78.4 | (70.1, 90.4) |
4 | 5 mg/kg | 8.4 | (6.2, 15.2) | |
5 | 7.5 mg/kg | 17.7 | (13.8, 20.4) | |
6 | 10 mg/kg | 10.1 | (7.1, 14.6) | |
60 | 3 | Control | 106.0 | (85, 120.7) |
7 | 5 mg/kg | 2.1 | (1.4, 2.8) | |
8 | 7.5 mg/kg | 1.9 | (1.3, 2.8) | |
9 | 10 mg/kg | 1.9 | (1.0, 2.1) | |