Group | DOXY | Necropsy day |
---|
30 (groups 2, 4, 5, 6) | 60 (groups 3, 7, 8, 9) |
---|
2, 3 | Control | 100 | 100 |
4, 7 | 5 mg/kg | 40 | 0 |
5, 8 | 7.5 mg/kg | 0 | 0 |
6, 9 | 10 mg/kg | 0 | 0 |
- Quantitative PCR was performed on DNA isolated from whole blood collected on the day of necropsy. Primers were designed to detect D. immitis 18S and Wolbachia ftsZ genes with SYBR green dye. Samples were categorized as negative for the presence of Wolbachia DNA if Ct values were ≥ 35 or no DNA was amplified