Fig. 1

The increased expression of SARM after T. gondii in vivo and in vitro. A-C, The qPCR for SARM mRNA was performed on T. gondii-infected KM mouse brains and age-matched noninfected brain homogenates from KM mouse brains in vivo. (A), T. gondii-infected HT22 cells and noninfected HT22 cells in vitro (B), T. gondii-infected BV2 cells and noninfected BV2 cells in vitro (C). Relative expression was normalized to actin expression. A n = 5 mice per group. B, C n = 3 per group. D–F, Western blot detection of SARM and GAPDH expression in mouse brains infected and noninfected with T. gondii in vivo (D), SARM and Tubulin expression in T. gondii-infected HT22 cells and noninfected HT22 cells in vitro at an MOI of 2 (E), and T. gondii-infected BV2 cells and noninfected BV2 cells at an MOI of 2 (F). Densitometric quantification of the SARM western blot was performed. The qPCR and western blot results represented at least three independent experiments. D, n = 5 mice per group. E and F, n = 3 per group. Data were shown as mean ± SD. ns, not significant, compared with control group; *P < 0.05, compared with control group; **P < 0.01, compared with control group; ***P < 0.001, compared with control group