Fig. 2

SARM did not affect cell proliferation. HT22 and BV2 cells were transiently transfected with 4 μg of SARM plasmids. At 24 hpi, cells were seeded into 96-well plates and cultured for 24 h. The EdU incorporation rate was calculated as the ratio of the number of EdU-incorporated cells to Hoechst 33342-stained cells. A, Transient overexpression of SARM in HT22 cells did not affect cell proliferation, scale bar represents 100 μm. n = 5 per group. B, Transient overexpression of SARM in BV2 cells did not affect cell proliferation, scale bar represents 50 μm. n = 3 per group. Data were shown as mean ± SD. ns, not significant