Fig. 3

Induction of cell apoptosis and ROS by SARM in HT22 and BV2 cells. A–C, SARM overexpression accelerated T. gondii-mediated cell apoptosis. HT22 cells were transiently transfected with 4 μg of SARM plasmids. After 24 h of SARM overexpression, the cells were co-cultured with T. gondii tachyzoites for 24 h, and cell apoptosis was measured by flow cytometry (A). HT22 cells were stably transfected with SARM and co-cultured with T. gondii tachyzoites for 24 h, cell apoptosis was measured by flow cytometry (B). BV2 cells were transiently transfected with 4 μg of SARM plasmids. After 24 h of SARM overexpression, the cells were co-cultured with T. gondii tachyzoites for 24 h, cell apoptosis was measured by flow cytometry (C). D and E, SARM overexpression generated T. gondii-mediated ROS. HT22 cells were transiently transfected with 4 μg of SARM plasmids after 24 h of overexpression of SARM and co-cultured with T. gondii tachyzoites for 24 h, and intracellular ROS was measured by flow cytometry (D). BV2 cells were transiently transfected with 4 μg of SARM plasmids after 24 h of overexpression of SARM and co-cultured with T. gondii tachyzoites for 24 h, intracellular ROS was measured by flow cytometry (E). n = 3 per group. Data are shown as mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.001