Fig. 4

SARM mediated cell apoptosis via the mitochondrial apoptosis pathway. A, HT22 cells were transiently transfected with 4 μg of SARM plasmids after 24 h of overexpression of SARM and co-cultured with T. gondii tachyzoites for 24 h; mitochondrial apoptosis pathway-related proteins were examined by western blot. B, The chart shows the quantification of relative protein expression (A). C, HT22 cells were stably transfected with SARM and co-cultured with T. gondii tachyzoites for 24 h, and mitochondrial apoptosis pathway-related proteins were examined by western blot. D, The chart shows the quantification of relative protein expression (C). E, BV2 cells were transiently transfected with 4 μg of SARM plasmids after 24 h of overexpression of SARM and co-cultured with T. gondii tachyzoites for 24 h, and mitochondrial apoptosis pathway-related proteins were examined by western blot. F, The chart shows the quantification of relative protein expression of (E). n = 3 per group. Data are shown as mean ± SD. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001