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Fig. 2 | Parasites & Vectors

Fig. 2

From: m5C methylation of mitochondrial RNA and non-coding RNA by NSUN3 is associated with variant gene expression and asexual blood-stage development in Plasmodium falciparum

Fig. 2

PfNSUN3 knockdown triggered alteration of the transcriptome of blood-stage parasites. a Upper: Schematic representation of the construction of the transgenic line PfNSUN3-Ty1-Ribo. Lower: PCR analysis of PfNSUN3-Ty1-Ribo lines (left). Western blot of PfNSUN3 protein with antibody against Ty1 for the PfNSUN3-Ty1-Ribo and PfNSUN3-HA-Ty1 lines. Aldolase was used as an internal control (right). b Growth curve assay of the PfNSUN3-Ty1-Ribo strain with or without culture GlcN (n = 3, bars indicate standard deviation [SD]). c–e Comparative transcriptome analysis of PfNSUN3-Ty1-Ribo versus WT clones at the ring, trophozoite, and schizont stages

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Parasites & Vectors

ISSN: 1756-3305

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