Acaricidal efficacy of fluralaner against Rhipicephalus microplus ticks under laboratory and field conditions in Brazil

Table 3 Mean counts of Rhipicephalus microplus females (size range: 4.5–8 mm) in cattle treated with a pour-on formulation containing fluralaner (2.5 mg/kg), on three farms

Days post-infection	Population 10 (LC50 = 0.415 µg/mL)^a					Population 14 (LC50 = 0.144 µg/mL)^b					Population 16 (LC50 = 0.481 µg/mL)^c
Days post-infection	Control	Treatment	Efficacy	p value	CV	Control	Treatment	Efficacy	p value	CV	Control	Treatment	Efficacy	p value	CV
0 (day of infection)	95.1a	95.6a		0.9515	11.47	19.7a	19.9a		0.8923	4.9	33.7a	33.43a		0.9613	9.55
+3	99.0a	0.0b	100	< 0.0001	16.45	22.7a	0.0b	100	< 0.0001	13.38	27.0a	0.0b	100	< 0.0001	30.37
+7	102.2a	0.0b	100	< 0.0001	18.11	24.6a	0.0b	100	< 0.0001	13.21	26.9a	0.0b	100	< 0.0001	22.89
+14	89.8a	0.0b	100	< 0.0001	16.42	29.4a	0.0b	100	< 0.0001	16.48	25.3a	0.0b	100	< 0.0001	13.33
+21	90.3a	0.0b	100	< 0.0001	19.42	30.2a	0.0b	100	< 0.0001	13.4	31.8a	0.0b	100	< 0.0001	4.23
+28	93.4a	0.0b	100	< 0.0001	16.93	37.0a	0.0b	100	< 0.0001	19.97	26.8a	0.0b	100	< 0.0001	6.51
+35	78.7a	0.0b	100	< 0.0001	23.66	41.4a	0.1b	99.8	< 0.0001	19.29	31.7a	0.0b	100	< 0.0001	8.63
+42	54.9a	2.5b	95.5	< 0.0001	28.1	41.0a	4.1b	90.1	< 0.0001	35.24	27.6a	1.4b	94.9	< 0.0001	22.01
+49	45.1a	7.0b	84.6	< 0.0001	31.39	41.5a	7.5b	82.1	0.0001	32.92	24.8a	4.80b	80.5	0.0020	47.49
+56	38.3a	24.5a	36.4	0.1597	26.08	39.4a	20.0b	49.7	0.0180	28.51	30.9a	12.60b	58.9	0.0024	35.27
Therapeutic efficacy (up to day +21)			100					100					100
Persistent efficacy (day +28 to day +42)			98.9					96.5					98.2

Mean followed by different letters, in the same column, for the same population, are significantly different at the 5% level
CV Coefficient of variation
^aPopulation 10: second highest LC50 (0.415 µg/mL)—obtained from farm located in Piracanjuba, GO, Brazil
^bPopulation 14: lower LC50 value (0.144 µg/mL)—obtained from farm located in Anápolis, GO, Brazil
^cPopulation 16: highest LC50 value CL50 (0.481 µg/mL)—obtained from farm located in São João da Boa Vista, SP, Brazil

ISSN: 1756-3305