Fig. 2

Identification of the HL-TRP channel. A The relationship of HL-TRP channel with other species from a maximum likelihood phylogeny; HL-TRP is marked with red triangles. B Immunofluorescence of HL-TRP in HEK293T; green fluorescence: HL-TRP; red fluorescence: Na⁺, K⁺-ATPase α1, cell membrane; blue fluorescence: cell nuclei; scale bar = 10 μm. C The expression of HL-TRP in the first pair of tick legs by in situ hybridization. Pink arrow and box mark Haller’s organ; green fluorescence: HL-TRP; blue fluorescence: cell nuclei; scale bar = 50 μm. D mRNA expression of the HL-TRP channel at different developmental stages. Different letters indicate significant differences (P < 0.05). E Transcription analysis of the HL-TRP channel in different tissues during an unfed stage. Different letters indicate significant differences (P < 0.05)