Fig. 1

Comparative analysis of two qPCR machines and bCUBE-qPCR-based standard graphs for detecting P. falciparum. A Correlation curve depicting the comparison of P. falciparum 18S rRNA gene detection in serially diluted DNA using bCUBE and laboratory-standard real-time qPCR. The correlation coefficient was calculated for twofold serially diluted P. falciparum DNA. The bCUBE qPCR Ct values are plotted on the x-axis and standard qPCR Ct values on the y-axis. B Standard curve showing the correlation of bCUBE Ct values with 10-fold serially diluted P. falciparum genomic DNA (20 ng to 0.2 pg). C Standard curve showing the correlation of bCUBE Ct values with 10-fold serially diluted P. falciparum-infected blood (from 5 × 10⁴ to 0.5 parasitized RBCs/μl). The slope of each line represents [−1/log₁₀ (PCR efficiency)] for a TaqMan probe assay. R² represents the correlation coefficient of a slope. Ct, cycle threshold