Fig. 2

Plasmodium falciparum infection prevalence in An. gambiae as detected by bCUBE qPCR versus microscopy. Bar graph depicting the P. falciparum infection prevalence (± SD) of oocysts (A) and sporozoites (B) at 9 and 15 days post-infectious blood meal in An. gambiae, respectively. Three independent experiments were conducted using An. gambiae mosquitoes infected with P. falciparum at low and high gametocytemia to establish various infection intensities. Statistical analyses were performed using the Mann–Whitney test. A standard correlation graph showing the detection of oocyst load in microscopy confirmed midgut oocyst samples (C) and twofold serially diluted salivary gland sporozoite samples (D) by bCUBE. R² represents the correlation coefficient of the slope, Ct the cycle threshold, and ns, not significant (P > 0.05)