Table 1 Primers and probes used to detect Plasmodium species targeting 18S rRNA genes (Shokoples et al. [21])
From: Off-grid field-deployable molecular diagnostic platform for malaria surveillance
Plasmodium spp. | Primer | Sequence (5′-3′) | Fluorescent label |
|---|---|---|---|
Simplex PCR | |||
P. falciparum | Fal-F | CCG ACT AGG TGT TGG ATG AAA GTG TTAA | FAM-MGBEQ |
Fal-R | AAC CCA AAG ACT TTG ATT TCT CAT AA | ||
Fal Probe | AGCAATCTAAAAGTCACCTCGAAAGATGACT | ||
Duplex PCR | |||
P. falciparum | Fal-F | CCG ACT AGG TGT TGG ATG AAA GTG TTAA | FAM-MGBEQ |
Fal-R | AAC CCA AAG ACT TTG ATT TCT CAT AA | ||
Fal Probe | AGCAATCTAAAAGTCACCTCGAAAGATGACT | ||
P. vivax | Viv-F | CCG ACT AGG CTT TGG ATG AAA GAT TTTA | HEX-MGBEQ |
Viv-R | AAC CCA AAG ACT TTG ATT TCT CAT AA | ||
Viv Probe | AGCAATCTAAGAATAAACTCCGAAGAGAAAATT | ||
Triplex PCR | |||
P. malaria | Mal-F | CCG ACT AGG TGT TGG ATG ATA GAG TAAA | FAM-MGBEQ |
Mal-R | AAC CCA AAG ACT TTG ATT TCT CAT AA | ||
Mal Probe | CTATCTAAAAGAAACACTCAT | ||
P. ovale | Ova-F | CCG ACT AGG TTT TGG ATG AAA GAT TTTT | HEX-MGBEQ |
Ova-R | AAC CCA AAG ACT TTG ATT TCT CAT AA | ||
Ova Probe | CGAAAGGAATTTTCTTATT | ||
P. knowlesi | Knw-F | CTA AAA TGC GCA CAA AGT CGAT | CY5-MGBEQ |
Knw-R | GCA GTT AAA ACG CTC GTA GTT GAA | ||
Knw Probe | CGGAGGCATCAGTTAT | ||