Table 2 Duplex bCUBE-qPCR-based detection of P. falciparum and P. vivax in mixed plasmid templates targeting Plasmodium 18S rRNA gene
From: Off-grid field-deployable molecular diagnostic platform for malaria surveillance
P. falciparum (18S rRNA) copy number | P. vivax (18S rRNA) copy number | Duplex bCUBE qPCR cycle threshold ± standard deviation | |
|---|---|---|---|
P. falciparum (FAM) | P. vivax (HEX) | ||
103 | 0 | 28.8 ± 0.07 | NTD |
104 | 0 | 25.7 ± 0.06 | NTD |
105 | 0 | 22.2 ± 0.14 | NTD |
106 | 0 | 19.2 ± 0.07 | NTD |
0 | 103 | NTD | 27.9 ± 0.21 |
0 | 104 | NTD | 24.4 ± 0.24 |
0 | 105 | NTD | 21.1 ± 0.01 |
0 | 106 | NTD | 18.0 ± 0.17 |
103 | 103 | 29.6 ± 0.14 | 28.7 ± 0.07 |
103 | 104 | 29.5 ± 0.07 | 25.8 ± 0.07 |
103 | 105 | 31.2 ± 0.99 | 22.3 ± 0.21 |
103 | 106 | 36.2 ± 0.57 | 18.7 + 0.21 |
104 | 103 | 26.3 ± 0.07 | 27.8 ± 0.29 |
105 | 103 | 22.7 ± 0.28 | NTD |
106 | 103 | 19.8 ± 0.14 | NTD |