Table 3 Triplex bCUBE-qPCR-based detection of P. malariae, P. ovale, and P. knowlesi in mixed plasmid templates targeting Plasmodium 18S rRNA gene
From: Off-grid field-deployable molecular diagnostic platform for malaria surveillance
P. malariae (18S rRNA) copy number | P. ovale (18S rRNA) copy number | P. knowlesi 18S rRNA) copy number | Triplex bCUBE qPCR cycle threshold ± standard deviation | ||
|---|---|---|---|---|---|
P. malariae (FAM) | P. ovale (HEX) | P. knowlesi (CY5) | |||
103 | 0 | 0 | 28.7 ± 0.04 | NTD | NTD |
104 | 0 | 0 | 24.6 ± 0.17 | NTD | NTD |
105 | 0 | 0 | 22.0 ± 0.05 | NTD | NTD |
106 | 0 | 0 | 18.4 ± 0.16 | NTD | NTD |
0 | 103 | 0 | NTD | 28.9 ± 0.57 | NTD |
0 | 104 | 0 | NTD | 25.3 ± 0.11 | NTD |
0 | 105 | 0 | NTD | 21.9 ± 0.04 | NTD |
0 | 106 | 0 | NTD | 18.7 ± 0.18 | NTD |
0 | 0 | 103 | NTD | NTD | 29.1 ± 0.25 |
0 | 0 | 104 | NTD | NTD | 25.6 ± 0.15 |
0 | 0 | 105 | NTD | NTD | 23.1 ± 0.68 |
0 | 0 | 106 | NTD | NTD | 18.7 ± 0.12 |
103 | 103 | 103 | 29.7 ± 0.30 | 29.7 ± 0.41 | 32.5 ± 0.67 |
103 | 103 | 104 | 30.0 ± 0.13 | 30.5 ± 0.19 | 29.0 ± 0.24 |
103 | 103 | 105 | 30.0 ± 0.17 | 30.0 ± 0.33 | 25.0 ± 0.15 |
103 | 103 | 106 | 32.7 ± 1.23 | 32.0 ± 2.40 | 22.0 ± 1.23 |
103 | 104 | 103 | 30.1 ± 0.20 | 27.0 ± 0.02 | 34.6 ± 1.06 |
103 | 105 | 103 | 32.8 ± 0.81 | 23.7 ± 0.05 | 37.7 ± 0.51 |
103 | 106 | 104 | 34.9 ± 0.27 | 20.2 ± 0.23 | NTD |
104 | 103 | 103 | 25.7 ± 0.50 | 29.8 ± 0.50 | 37.4 ± 0.50 |
105 | 103 | 103 | 23.6 ± 0.08 | 30.3 ± 2.81 | NTD |
106 | 103 | 103 | 19.5 ± 0.09 | NTD | NTD |