External quality assessment for molecular detection of sand fly-borne phleboviruses circulating in the Mediterranean Basin

Table 4 Results from the Toscana virus- and Sandfly fever Sicilian virus-specific reverse-transcriptase PCR assays and from the Pan-generic real-time PCR assays, from the different participating laboratories.

Laboratory identity number	TOSV (positive n=2)	TOSV (Lineage A and B), Ct	%^b	Negative (n=6)	Pan-SFSV (positive n=1)	SFSV Ct^a	%^b	Negative (n=7)	Pan-Phlebovirus (positive n=6)	Detected phleboviruses^c	%^b	Negative (n=2)	Total^b
Reference Laboratory	2	TOSV A 26.2 TOSV B 26.4	100	6	1	26.0	100	7	6	PUNV,ARBV, MASV, SFSV, TOSV A and B	100	2	100%
1	2	TOSV A 26.6 TOSV B 27.1	100	6	1	26.0	100	7	6	PUNV,ARBV, MASV, SFSV, TOSV A and B	100.0	2	100.0%
2	2	TOSV A 28.9 TOSV B 28.1	100	6	1	28.6	100	7	5	PUNV,ARBV, MASV, SFSV, TOSV A	83.3	3	83.3%
3	2	TOSV A 32.8 TOSV B 32.1	100	6	1	29.2	100	7	2	PUNV,ARBV,	33.3	6	77.7%
4	2	TOSV A 25.9 TOSV B 26.1	100	6	1	23.3	100	7	3	PUNV,ARBV, MASV	50.0	5	83.3%
5	2	TOSV A 30.0 TOSV B 30.0	100	6	1	31.0	100	7	5	PUNV,ARBV, MASV, SFSV, TOSV A	83.3	3	94.4%
6	2	TOSV A 31.8 TOSV B 33.4	100	6	1	28.8	100	7	5	PUNV,ARBV, MASV, SFSV, TOSV A	83.3	3	94.4%
7	2	TOSV A 31.2 TOSV B 33.5	100	6	1	29.7	100	7	3	PUNV,ARBV, MASV	50.0	5	83.3%
8	2	TOSV A 28.9 TOSV B 29.5	100	6	1	27.1	100	7	5	PUNV,ARBV, MASV, SFSV, TOSV A	83.3	3	94.4%
9	2	TOSV A 31.0 TOSV B 32.0	100	6	1	30.0	100	7	3	PUNV,ARBV, MASV	50.0	5	83.3%

SFSV Sandfly fever Sicilian virus. For definitions of ARBV, MASV, PUNV and TOSV, see Table 1
^aThe specific RT-qPCR assay outcomes are included as cycle threshold (Ct) values
^bThe accuracy percentage was calculated for each individual assay and for the combined results (Total column)

ISSN: 1756-3305