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Table 3 Individual results of tick counts and Babesia diagnosis

From: Efficacy of an oral combination of afoxolaner and milbemycin oxime for the prevention of transmission of Babesia canis by Dermacentor reticulatus ticks to dogs

Group1

#

Live (dead) attached tick count2

Original diagnosis of canine babesiosis

Scheduled bioanalyses of Babesia canis

Day of diagnosis, clinical signs of babesiosis, and/or rectal temperature of > 39.5 °C

Blood smear

PCR

IFA

PCR

Day 7

Day 34

Day 213

Day 28

Day 423

Day 56

Day 21

Day 28

Day 42

Day 56

Control 1

1

25

–

Day 9:

Lethargic

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

2

37

–

Day 9:

39.8 °C

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

3

13

–

Day 9:

40.6 °C

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

4

44

–

Day 8:

40.2 °C, lethargic

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

5

41

–

Day 8:

40.0 °C, lethargic

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

6

44

–

Day 8:

39.8 °C, lethargic

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

7

42 (2)

–

Day 9:

40.1 °C

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 1

8

42

–

Day 9:

39.9 °C, dark urine

Positive

Positive

Positive

–

–

–

Negative

–

–

–

Control 2

1

–

27

Day 36:

Lethargic

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

2

–

29

Day 37:

Dark urine

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

3

–

25

Day 35:

39.7 °C

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

4

–

40

Day 36:

40.2 °C

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

5

–

34

Day 35:

Lethargic

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

6

–

23

Day 36:

Lethargic, dark urine

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

7

–

26

Day 35:

39.6 °C

Positive

Positive

–

–

Positive

–

–

–

Negative

–

Control 2

8

–

39

Day 36:

40.0 °C

Positive

Positive

–

–

Positive

–

–

–

Negative

–

IVP

1

0

0

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

2

0 (2)

0 (5)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

3

0 (3)

0 (3)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

4

0 (3)

0 (4)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

5

0 (1)

0 (2)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

6

0

0 (2)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

7

0 (5)

0 (1)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

IVP

8

0 (4)

0 (1)

No abnormal sign or rectal temperature

–

–

Negative

Negative

Negative

Negative

Negative

Negative

Negative

Negative

  1. 1Control 1 dogs were infested on day 1 with 50 (± 2) D. reticulatus with a B. canis infection rate of 23%
  2. Control 2 dogs were infested on day 1 with 50 (± 2) D. reticulatus with a B. canis infection rate of 23%
  3. Treated group: dogs were treated on day 0 with NexGard Spectra® at a mean afoxolaner dose of 2.75 mg/kg and were infested on days 1 and 28 with 50 (± 2) D. reticulatus with a B. canis infection rate of 23%
  4. 2This column describes the counts of live (and dead) attached ticks. Otherwise, no live free ticks were found, and three dead free ticks were found on day 7 and day 34, each time in the treated group
  5. 3All dogs from control group 1 and control group 2 were removed from the study on days 21 and 42, respectively, and no further evaluation was performed as they had been confirmed positive for B. canis by IFA analysis