Table 1 Primers, target genes, and PCR conditions used in this study
Pathogen | Target gene | Primer (nucleotide sequence 5′-3′) | Method | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
Hepatozoon spp. | 18S rDNA | H14Hepa18SFw: GAAATAACAATACAAGGCAGTTAAAATGCT H14Hepa18SRv: GTGCTGAAGGAGTCGTTTATAAAGA | PCR | 620 | [40] |
Cytauxzoon spp. | ITS2 | C. felis F: TGAACGTATTAGACACACCACCT C. felis R: TCCTCCCGCTTCACTCGCCG | PCR | 430 | [41] |
Babesia spp., Hepatozoon spp. | 18S rDNA | Piroplasmid-F: CCAGCAGCCGCGGTAATTC Piroplasmid-R: CTTTCGCAGTAGTTYGTCTTTAACAAATCT | PCR | 350–400 | [42] |
Piroplasmids | 18S rDNA | GF2: GTCTTGTAATTGGAATGATGG GR2: CCAAAGACTTTGATTTCTCTC | PCR | 560–610 | [43] |
Leishmania spp. | kDNA minicircle | LEISH-1: AACTTTTCTGGTCCTCCGGGTAG LEISH-2: ACCCCCAGTTTCCCGCC Probe: FAM-AAAAATGGGTGCAGAAAT | qPCR | 120 | [44] |
Filarioids | cox1 | NTF: TGATTGGTGGTTTTGGTAA NTR: ATAAGTACGAGTATCAATATC | PCR | 648 | [45] |