Fig. 1

Titres in mosquito bodies and saliva after CHIKV and VLA1553 blood meals. A Mosquito body virus titres shown for CHIKV and VLA1553 at blood meal virus titres of ~ 7–8 log₁₀CCID₅₀/mL measured for series 1 experiments and body virus titres measured for series 2 experiments at descending blood meal virus titres of VLA1553. The blood meal virus titres shown in brackets on the x axis are the mean of three independent experiments (Table 2). The body virus titres for individual mosquitoes from the three independent experiments are shown on the y axis. ND, not detected; detection limit ~ 2 log₁₀CCID₅₀ (dashed line). Horizontal black lines represent mean titres, with ND set at zero. Statistics by Mann–Whitney U exact tests. B Mosquito saliva virus titres shown for CHIKV and VLA1553 at blood meal virus titres of ~ 7–8 log₁₀CCID₅₀/mL measured for series 1 experiments and saliva virus titres measured for series 2 experiments at descending blood meal titres of VLA1553. ND, not detected; saliva virus titre detection limit ~ 5.3 log₁₀CCID₅₀/mL saliva (dashed line). Statistics by Mann–Whitney U exact test. C Correlation between the percentage of mosquitoes with detectable VLA1553 present in the mosquito bodies versus the blood meal virus titre using the full data set in Table 2. D Correlation between the mean percentage of mosquitoes with detectable VLA1553 present in the mosquito bodies versus the mean blood meal titre (Table 2). E Correlation between the percentage of mosquitoes with detectable VLA1553 present in the mosquito saliva versus the blood meal virus titre using the full data set in Table 2. F Correlation between the mean percentage of mosquitoes with detectable VLA1553 present in the mosquito saliva versus the mean blood meal virus titre (Table 2)