Assessment of the transmission of live-attenuated chikungunya virus vaccine VLA1553 by Aedes albopictus mosquitoes

Table 3 Transmission of CHIKV or VLA1553 from A. albopictus mosquitoes to Irf3/7^−/− mice

Group	Experiment	No. of fed	Body positive	Saliva positive	No. of mosquitoes fed on mice		No. of mice with viraemia*
Group	Experiment	mosquitoes	(%)	(%)	Total	Per mouse^#	No. of mice with viraemia*
VLA1553	1.1	42	40.5	0	13	5 + 3 + 5	0/3
	1.2	20	65.0	6.7	4	1 + 1 + 2	0/3
CHIKV	1.1	41	97.6	41.4	11	4 + 4 + 3	3/3
	1.2	26	96.2	21.0	6	2 + 2 + 2	0/3

^*Read out for infection was viraemia measurements from day 0 to day 16 by CCID₅₀ assays [43] (see Fig. 2). ^#One mouse per container of CHIKV or VLA1553-fed mosquitoes (13–15 per container), n = 3 mice per group
Experiment: series 1 with CHIKV versus VLA1553 using mosquitoes from repeat experiments 1.1. and 1.2

ISSN: 1756-3305