Table 2 List of primer sets used per species with primer details
Species | Primer set (reference) | Primer sequence (5′–3′) | Ta (°C) |
|---|---|---|---|
Aedes aegypti | F: SCTL2-J-3037 [87] | ATGGCAGATTAGTGCAATGA | 47 |
R: Ae-COII-R [32] | GATTTAAGAGATCATTACTTGC | ||
Aedes taeniorhynchus | F: SCTL2-J-3037 | ATGGCAGATTAGTGCAATGA | 47 |
R: Ae-COII-R | GATTTAAGAGATCATTACTTGC | ||
Culex nigripalpus | F: SCTL2-J-3037 | ATGGCAGATTAGTGCAATGA | 47 |
R: Cul-COII-R (†) | GRTTTAAGAGAYCAKTACTTGC | ||
Culex quinquefasciatus | F: SCTL2-J-3037 | ATGGCAGATTAGTGCAATGA | 45 |
R: TK-N-3785 [87] | GTTTAAGAGACCAGTACTTG | ||
Deinocerites sp. | F: SCTL2-J-3037 | ATGGCAGATTAGTGCAATGA | 47 |
R: Cul-COII-R (†) | GRTTTAAGAGAYCAKTACTTGC | ||
Haemagogus chrysochlorus | F: SCTL2-J-3037 | ATGGCAGATTAGTGCAATGA | 47 |
R: Cul-COII-R (†) | GRTTTAAGAGAYCAKTACTTGC |